In vitro evaluation of potato plantlets raised from true seeds for drought tolerance

Amini, Kobra and Asghari-Zakaria, Rasool and Asghari, Ali and Seyed Sharifi, Rauf (2010) In vitro evaluation of potato plantlets raised from true seeds for drought tolerance. Other thesis, University of Mohaghegh Ardabili.

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Abstract

In order to in vitro evaluation of potato genotypes to drought tolerance, an experiment was done as a factorial experiment with tow factors based on completely randomized design in 6 repeat, for the first experiment and four replications for the second experiment. The first factor include single node explants derived from true potato seeds of six genotype (Agria, Caeser, Geranula, CIP-994001, Satina and Diyamund) and the second factor in the first experiment included six concentrations of polyethylene glycol 6000 (0, 2, 4, 6, 8 and 10 mM), and the second experiment five concentrations mannitol (0, 0.1, 0.2, 0.3 and 0.4 M) of that added in MS medium. Root and shoot length, fresh and dry weights of root and shoot, number of roots, shoot and leaf and root to shoot ratio based on length and dry weight were measured in plantlet grown in medium with different concentration of PEG and mannitol. All of traits decreased with increase in the concentration of PEG and mannitol except for root to shoot ratios based on length and dry weight unit. In the first experiment differeces between genotypes and interaction of genotypes × PEG concentration were significant (P≤0/01) at all of traits. At stress levels 2, 4, 6 and 8 mM PEG, the plantlets drived from true seeds of CIP-994001 and Diyamund genotypes had respectively the highest and lowest Root and shoot length, fresh and dry weights of root. Also, CIP-994001 genotype in term of shoot fresh weight in level stress 2, 4 and 8 mM PEG, in term of shoot dry weight in level stress 2, 4 and 6 mM PEG was tolerant genotype. Calculating tolerance index (as the percentage of decreasing of traits at different concentration compared with control) showed that in general, the plantlets drived from true seeds of Satina and caesar genotypes in mild stress conditions (6 mM PEG) and severe stress (10 mM PEG) were identified respectively of the tolerant and sensitive genotypes. In the second experiment Differeces between genotypes and interaction of genotypes × PEG concentration were significant (P≤0/01) at all of traits except number of shoot. At 0.3 M concentration of mannitul, the plantlets drived from true seeds of CIP-994001 and Agria genotypes had significantly higher Root and shoot length, root dry weight and shoot dry weight than the other three genotypes. At 0.4 M concentration of mannitul, only Satina genotype produced roots. In general, According to the TOL index in the mild, CIP-994001 and Satina genotypes were tolerant and in the severe stress conditions none of genotypes did not show a high tolerance.

Item Type: Thesis (Other)
Persian Title: ارزيابي درون¬شيشه¬اي گياهچه¬هاي حاصل از بذر حقيقي سيب¬زميني از نظر تحمل خشکي
Persian Abstract: به منظور ارزيابي درون شيشه¬اي ژنوتيپ¬هاي سيب‌زميني براي تحمل به خشكي آزمايشي به صورت فاکتوريل در قالب طرح کاملاً تصادفي در 6 تکرار در آزمايش اول و چهار تکرار براي آزمايش دوم اجرا شد. فاکتور اول عبارت بود از: گياهچه¬هاي حاصل از بذور حقيقي چند ژنوتيپ سيب¬زميني (آگريا، کايزر، CIP-994001، گرانولا، ساتينا و دياموند) و فاکتور دوم در آزمايش اول، سطوح مختلف PEG (صفر، 2، 4، 6، 8 و 10 ميلي¬مولار) و در آزمايش دوم سطوح مختلف مانيتول (صفر، 1/0، 2/0، 3/0 و 4/0 مولار) را شامل می¬شد. سطوح مختلف تنش در محيط كشت MS اعمال و صفات طول ريشه و ساقه، وزن تر و خشک ريشه و اندام¬هاي هوايي، تعداد ريشه، ساقه و برگ و نسبت ريشه به ساقه بر اساس واحد طول و واحد وزن خشک اندازه¬گيري شد. با افزايش غلظت PEG و مانيتول تمامي صفات مورد مطالعه به جز نسبت طول ريشه به ساقه و نسبت وزن خشک ريشه به ساقه کاهش يافت. در آزمايش اول پاسخ ژنوتيپ¬ها و اثر متقابل ژنوتيپ × تنش براي همه صفات معني¬دار شد (01/0p≤). در سطوح تنش 2، 4، 6 و 8 ميلي¬مولار PEG، ژنوتيپ 994001CIP- و دياموند از لحاظ صفات طول ريشه، طول گياهچه، وزن خشک ريشه و وزن تر ريشه به ترتيب بيشترين و کمترين ميزان را داشتند و هم¬چنين ژنوتيپ 994001CIP- از لحاظ وزن تر ساقه در سطوح تنش 2، 4 و 8 ميلي¬مولار PEG، و از لحاظ وزن خشک ساقه در سطوح تنش 2، 4 و 6 مولار PEG، ژنوتيپ متحمل بود. محاسبه شاخص تحمل (درصد کاهش نسبت به شرايط شاهد) نشان داد که در مجموع، گياهچه‌هاي حاصل از بذور حقيقي ژنوتيپ¬هاي ساتينا و کايزر در شرايط تنش ملايم (6 ميلي¬مولار PEG) و تنش شديد (10 ميلي¬مولار PEG) به ترتيب به عنوان ژنوتيپ¬هاي متحمل و حساس شناخته شدند. در آزمايش دوم پاسخ ژنوتيپ¬ها و اثر متقابل ژنوتيپ × تنش براي همه صفات به جز تعداد ساقه معني¬دار شد (01/0p≤). در غلظت 3/0 مولار مانيتول، طول گياهچه، طول ريشه وزن خشک ريشه و وزن خشک ساقه ژنوتيپ¬هاي 994001CIP- و آگريا به طور معني¬دار بزرگتر از ژنوتيپ¬هاي ديگر شدند. در غلظت 4/0 مولار مانيتول تنها ژنوتيپ ساتينا ريشه توليد کرد. در مجموع، ميزان تحمل ژنوتيپ¬ها بر اساس شاخص TOL در شرايط تنش ملايم ژنوتيپ¬هاي CIP-994001 و ساتينا متحمل معرفي شدند و در شرايط تنش شديد هيچ كدام از ژنوتيپ¬ها تحمل بالايي از خود نشان ندادند.
Supervisor:
SupervisorE-mail
Asghari-Zakaria, RasoolUNSPECIFIED
Asghari, AliUNSPECIFIED
Advisor:
AdvisorE-mail
Seyed Sharifi, RaufUNSPECIFIED
Subjects: Faculty of Agricultural Sciences & Natural Resources > Department of Agronomy & Plant Breeding
Divisions > Faculty of Agricultural Sciences & Natural Resources > Department of Agronomy & Plant Breeding
Divisions: Subjects > Faculty of Agricultural Sciences & Natural Resources > Department of Agronomy & Plant Breeding
Faculty of Agricultural Sciences & Natural Resources > Department of Agronomy & Plant Breeding
Date Deposited: 09 Jul 2019 07:41
Last Modified: 09 Jul 2019 07:41
URI: http://repository.uma.ac.ir/id/eprint/7592

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