Investigation of the effects of baclofen on RFamide- related peptide and kisspeptin gene expressions in polycystic ovary syndrome

Rahimi Rick, Elahe (2019) Investigation of the effects of baclofen on RFamide- related peptide and kisspeptin gene expressions in polycystic ovary syndrome. Masters thesis, University of Mohaghegh Ardabili.

Text نهایی رحیمی.pdf Download (852kB)

Abstract

Research Aim:Gamma amino butyric acid (GABA) is one of the most important inhibitory neurotransmitters in the central nervous systemand exerts an inhibitory effect on GnRH neurons. Polycystic ovary syndrome (PCOS) is one of the most common metabolic and reproductive disorders in women. Arginine-phenylalanine-amide-relatedpeptide(RFRP3) and kisspeptin exert a stimulatory and inhibitory effect on the reproductive axis activity respectively. There is a direct relationship between PCOS and increased GnRH/LH hormones secretion. Changes in the level of kisspeptin and and RFRP-3 synthesis and decreased GABA release have been shown in PCOS.According to the abovein the present study, the effects of baclofen (GABAB receptor agonist) were evaluated on the mean relative expression of KiSS1 and RFRP3 genes in intact and PCOS rats. Research method: Twenty female Wistar rats weighing 180-200g were divided into four groups (n=5 in each group). Fifteen rats in three groups received intramuscular injection of 2 mg estradiol valeratein 0.5ml sesame oil in order to induce PCOS. The PCOS rats received intraperitoneal injection of saline or baclofen at a dose of 5 or 10mg/kg BW for 14 consecutive dyas. Five intact ras as a control group received intraperitoneal injection of saline for 14 consecutive dyas. One day following the last injection, the hypothalamus specimens were isolated and the mean relative gene expression of KiSS1 and RFRP3 was determined by real-time polymerase chain reaction (RT-PCR).The data were analyzed by T-test, one way ANOVA test and Tukey post hoc test by using SPSS software. The results were reported with a significant level of P≤0.05. Findings: The mean relative gene expression of KiSS1 was significantly increased in PCOS group compared to intact rats. The mean relative gene expression of RFRP3 did not significantly decrease in PCOS group compared to intact ones. Different doses of baclofen caused a significant decrease in mean relative gene expression of KiSS1anda significant increase in mean relative gene expression of RFRP3 in PCOS rats compared to PCOS control group. Conclusion: The GABAergic signaling pathway may be involved in the regulation of GnRH/LH hormones secretion via inhibiting or stimulating the synthesis of intra-hypothalamic neuropeptides upstream GnRH neurons in PCOS patients

Actions (login required)

View Item