Preperation of Silver Nanoparticle by AGreen Method and The Antisense Mcl1 Conjugated Form of The Nanoparticle, As ACytotoxic Agent Against MCF7 Cancerous Cell line

Ghorbanipour, Soheila (2019) Preperation of Silver Nanoparticle by AGreen Method and The Antisense Mcl1 Conjugated Form of The Nanoparticle, As ACytotoxic Agent Against MCF7 Cancerous Cell line. Masters thesis, University of Mohaghegh Ardabili.

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Abstract

Introduction: Recently, research in gene therapy has been focused in the cancer treatment and inhibition. Fabrication of a suitable carrier is main problem of an efficient gene delivery system and variousresearch seeks to solve the problem. The AgNp, which has been prepared by green methods, shows unique properties including biocompatibility, biodegradability and ignorable cytotoxicity, for these reason, has attracted to attention of many researchers. Aim of research: The main objective of this study is preparation of a Nanoparticle by the green methods which can be used as a vehicle for transferring of the antisense oligonucleotide to the cancerous cells. Methods:In this investigation, the AgNps were prepared by various green methods, afterward, were conjugated to an antisense oligonucleotide. The nanoparticles properties were analyzed and their biological effects against the cancerous cell line as well as the wound healing activity were studied. Finding:The data showed that AgNps were efficiently prepared by the green methods and the modified antisense oligonucleotide was conjugated to the nanoparticle. Physical properties of the AgNps were recorded. The cytotoxic activity of the oligonucleotide-conjugated AgNp was considerable, whereas, AgNp did not showed significant cytotoxicity, but efficient wound healing effects was showed by the AgNp. Discution: The previous published data revealed that inhibition of Mcl1 expression led to induction of apoptosis in the cancerous cell lines. In this research AgNp was used as vehicle to delivery of Mcl1 antisense oligonucleotide to the cancerous cell line lines and the increment of cell cytotoxicity was proved.

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