Survey on the relationship between ACTN3 Gene polymorphism and the power performance of male students aged 8_12 years old in Ardabil city

vakili, Saeede (2019) Survey on the relationship between ACTN3 Gene polymorphism and the power performance of male students aged 8_12 years old in Ardabil city. Masters thesis, University of Mohaghegh Ardabili.

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Abstract

Introduction and goal: Recently, several polymorphisms have been identified that are significantly associated with exercise performance. In the mean while, Gene ACTN3 has been introduced as a good representative of athletic talent that can be effective in guiding children to relevant sports. Therefore, the aim of the present study was to investigate theACTN3-gene polymorphism with power function of male student’s 8_12 years old in Ardabil city. Methodology: Subjects included 86 male students’ 8_12 years old from Ardabil city. Measured exercise performance included finger strength,endurance of the muscles of the fingers, back and leg muscles strength, abdominal muscle endurance, shoulder belt muscle endurance strength, cardio-respiratory fitness, and speed measured by field method.ACTN3 gene polymorphisms were determined and compared using polymerase(Tetra_ARMs PCR) reaction and direct sequencing of DNA through salivary samples.one way ANOVA and J2 tests were used to investigate the relationship between spotr function and ACTN3 gene polymorphism. Findings: The results showed a significant difference in the frequency of ACTN3 gene polymorphism in subjects with the highest frequency in RX, RR and XX genotypes. (RR-29, XX-3 and RX-54person)(P=0.001) One-way ANOVA test showed no significant difference in performance indices between (RR, XX, RX) polymorphisms ;( P>0.05) The result of J2 test in examining the relationship between ACTN3 gene polymorphism and students sport performance at three levels(low,medium,high)showed a significant relationship in aerobic endurance test result.(P=0.02);there was no significant difference in other performance indices.(P>0.05) Also the best genotypes were fingerprint test(RR-24.1% and RX-27.8%), back and leg muscle strength(RR-34.5% and RX-20.9%), finger muscle endurance(XX-33.3% and RX-27.8%), shoulder belt muscle endurance(XX-33.3% and RX-24.9%), abdominal muscle endurance(XX-22.2% and RR-27.6%), flexibility(XX-27.6% and RR-22.2%), aerobic endurance(XX-66.7% and RR-31.0%), speed(XX-33.3% and RX-27.7%) and power(RR-37.9% and RX-18.5%). Conclusion:the results showed the superiority of the RX(54 person) and RR(29 person)genotypes in Ardabil student’s 8-12 years old ACTN3 gene.subjects with genotype RR also had better performance in power,back and leg muscles strength,cardiopulmonary fitness,abdominal muscle endurance,flexibility and finger muscle strength,respectively.subjects with genotype RX had better performance in finger muscle endurance,finger muscle strength,endurance speed,shoulder belt muscles,flexibility,back and leg muscles strength,respectively,which earrants further.

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