Investigation of the Effects of Silver Nanoparticles Using Galega officinalis on Hematology and Serum Levels of Liver Enzymes in Alloxan Induced Diabets in Rat Model

Azimi kodeh kahriz, Fariba (2019) Investigation of the Effects of Silver Nanoparticles Using Galega officinalis on Hematology and Serum Levels of Liver Enzymes in Alloxan Induced Diabets in Rat Model. Masters thesis, University of Mohaghegh Ardabili.

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Abstract

Research Aim: Galega officinalis is one of the best medicinal plants for treatment of diabetes due its component similarity to metformin. Chronic diabetes is associated with damage of liver and kidney functions, changes of hematological parameters including decreasing the white blood cells(WBC), platlets and dysfunction of red blood cells(RBC). Silver nanoparticles are important components in biological studies due to their antioxidant, antibacterial, antihyperglysemic properties and so on. Green synthesis of silver nanoparticles using plants extrats in addition to the lower cost of the synthesis process, results in smaller nanoparticles and bettere performance than chemichal synthesis. In the present study, for the first time the effects of silver nanoparticles synthetized using aerial parts of Galega officinalis were determined on hematological parameters and serum levels of liver enzymes in diabetic rats. Research method: In the present study thirty male Wistar rats in six group(n= 5 in each group) weighing 180- 200 g were used. Intact or alloxan monohydrate(150mg/Kg) -induced diabetic rats receieved intraperitoneal injection of saline or 2/5mg/Kg silver nanoparticles synthetized by Galega officinalis extract or chemichal method for 14 days respectively. One day following the last injection of drugs, Blood samples were collected in overnight fasted rats. Mean serum concentration of glucose, urea, creatinine, albumin, alanine amino transferase(ALT), aspartate amino transferase(AST), gamma glutamil transpeptidase GGT(ᵞGT) and alkaline phosphatase(ALP) were determined by spectrophotometry using global 240 biochemistry analyzer. glycated hemoglobin(HbA1C) percentage was determined by Cobas Mira analyzer. Hematological parameters were determined by using XS800i Sysmex automated hematology analyzer. The data were analyzed by one way- ANOVA test followed by post hoc Duncan test and SPSS software. Findings: Mean serum levels of glucose and urea significantly decreased in diabetic rats receiving chemichal or green silver naoparticles compared to diabetic ones. Mean serum levels of ALT and AST enzymes significantly decreased in diabetic rats receiving green silver naoparticles compared to diabetic ones. Mean serum levels of ALT enzyme did not significantly decrease in diabetic rats receiving chemichal silver naoparticles compared to diabetic ones. Mean serum levels of AST enzyme significantly increased in diabetic rats receiving chemichal silver naoparticles compared to diabetic ones. Mean hemoglubin concentration and hematocrit percentage did not significantly increase in diabetic rats receiving green silver naoparticles compared to diabetic ones. Mean serum levels of GGT( ᵞGT), ALP, and AST enzymes, mean white blood cells(WBC) number and HbA1C percentage significantly decreased in diabetic rats receiving chemichal or green silver naoparticles compared to diabetic ones. Mean serum levels of albumin significantly increased in diabetic rats receiving chemichal or green silver naoparticles compared to diabetic ones. The decrease of mean serum level of creatinin, the increase of mean red blood cells(RBC) and platlets number were not statistically significant in diabetic rats receiving chemichal or green silver naoparticles compared to diabetic ones. Conclusion: Both chemical and green synthetized silver nanoparticles caused effective decrease in blood glucose levels of diabetic rat. However, silver nanoparticles synthetized using Galega officinalis extraxt may be more protective than chemical synthetized one in regulation liver function. It may decrease the serum levels of liver enzymes partlty via preventing hepatocyes damage in diabetes. Also, it may be effective in the treatment of cardio- vascular disorders associated with cronic diabetes by improwing the hematological parameters.

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