Evaluation of Biological Activity of Helicobacter pylori vacA c Region and Its Association with cagPAI Genes in Development of Gastric Adenocarcinoma

Bakhti, Seyedeh Zahra (2021) Evaluation of Biological Activity of Helicobacter pylori vacA c Region and Its Association with cagPAI Genes in Development of Gastric Adenocarcinoma. Doctoral thesis, Faculty of Science.

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Abstract

Research Aim: Research Aim: Iran is a high-risk country for cardia gastric adenocarcinoma (CGA) in Central Asia. Helicobacter pylori possesses virulence genes that are involved in the pathogenesis of the bacterium. VacA cytotoxin of H. pylori induces vacuolation and cell death, and plays a critical role in the development of gastric cancer (GC). The vacA c1 genotype, corresponding to a deletion variant (del-LVGVQ) at the carboxyl terminus of the p55 domain, is strongly associated with GC, but its functional role is still unknown. We aimed to i) find any associations between the vacA alleles and cagA status, ii) determine the frequency of all five-genotype combinations of the vacA alleles with and without cagA gene, and their associations with GC, iii) examine the impact of vacA c genotypes, cagA status, and possible genotype combinations on the risk of CGA and non-cardia gastric adenocarcinoma (NCGA), iv) examine the associations of multiple H. pylori cagPAI genotypes (i.e. cagH, cagL, cagG, and orf17) with the risk of CGA, NCGA, and different histological types of GA in Iran, as well as v) evaluation of biological Activity of vacA c Region in Human Gastric Adenocarcinoma AGS Cells.

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