Evaluation Of Enhancement factors Effects on osteogenic differentiation of hMSC on the nanofiberous scaffold

Rahmani, Farzaneh (2017) Evaluation Of Enhancement factors Effects on osteogenic differentiation of hMSC on the nanofiberous scaffold. Masters thesis, Faculty of saience.

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Abstract

Research Aim: To produce scaffold whit high strength and biocompatibility and biodegradability the electro spinning as one of the tissue engineering approaches was investigated in this study. Tissue engineering is the laboratory development and growth of the cells for replacing or repairing the damaged parts of body. Research method: It is important that the fabricated fiber to have not only ability of supporting from growth and differentiation of stem cell ,but also biocompatibility and biodegradability .Electro spinning is a process that produce fiber with less than micron diameter and scaffolds with high porosity similar to the natural extracellular matrix that was studied.in the process of electro spinning polymer solution in the syringe convert to the mentioned fiber with certain rate and distance from collector in the presence of electric current. Findings: In this paper, composite scaffold of PCL/MWCNT/n-HA was studied. The finding shows that scaffold with CNT has mechanical strength better than PCL polymer without any added particle. The presence of particle of n-HA makes the scaffold resemble to matrix of bone tissue and probably accelerate the osteogenic differentiation. In SEM pictures it was seen that fabricated fiber had size between 20-100 nm and was well distributed and had good uniformity of Ca and P ions on that’s surface. Conclusion: . According to this study, adding the other material to structure can accelerate the osteogenic differentiation and produce the suitable scaffold for bone tissue engineering.

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