Effect Of Aerobic Interval Versus Continuous Training On Soleus Muscle Apoptosis of Male Rats

Asgharpour arshad, Masoud and Siahkouhian, Marefat and bolboli, Lotfali and Jafari, Afshar and Sheykhzadeh hesari, Farzam (2016) Effect Of Aerobic Interval Versus Continuous Training On Soleus Muscle Apoptosis of Male Rats. Doctoral thesis, university of Mohaghegh Ardabili.

Text (تاثیر دو شیوه‌ی تمرینی تداومی و تناوبی هوازی بر آپوپتوز عضله‌ی نعلی موش‌های صحرایی نر) Masoud Asgharpour arshad.pdf Download (356kB)

Abstract

Background and objectives: Apoptosis is a protective cell process which plays important role in development and homeostasis of natural tissue and the emergence of diseases. In natural conditions this leads to elimination of old, damaged, waste and harmful cells and is essential for tissue repair. Apoptosis plays an important role in diseases which impairs tissue performance including muscle atrophy. Some evidences indicated that exercise may affect a number of apoptosis related signaling pathways in skeletal muscles. Thus, the present study aimed to determine the effect of two methods of continuous and interval aerobic exercise on soleus muscle apoptosis in male rats. Material and methods: Present study was multi group with control group experimental design which conducted on 38 three months old male rats. Subjects randomly were divided in to four homogenous groups including three months old base control (N=8), six months old control (N=10), continuous (N=10) and interval (N=10) exercise training. Training groups were participated in two continues and interval aerobic program for 12 weeks. Surgeries were done on three month old rats of bace control group before beginning of exercise protocol and on the subjects of other three groups after three months of training and following 48 hours of last training session, and then protein expression was evaluated. Data were evaluated by Real Time-PCR and the Caspase-3, Bcl-2 and Bax of soleus muscle were analyzed using the one way ANOVA and Bonferroni post hoc test (P< 0.05). Results: The findings showed that there is a significant difference between control and training groups in Bcl-2 (p<0.01) and the Bax to Bcl-2 ratio (p<0.05). Gene expression of Bcl-2 in training group was higher than control (it was significant in continuous training but insignificant in interval training group). Bax to Bcl-2 ratio were significantly lower in both training groups than control group, but there were no significant difference in Bax and Caspase-3 gene expressions between groups (p>0.05). However, the Bax gene expression of interval training group were 44 and 46 percent lower compared with control and continuous training groups. The Caspase-3 gene expression in continuous training group was 53 percent higher than control and in interval training group 21 percent lower than control group. Conclusion: It seems that 12 weeks of aerobic training specially the continuous one have a significant effect on the increase of mitochondrial anti-apoptotic proteins of soleus muscle. However, regarding the significant low levels of Bax to Bcl-2 ratio in training group than control group and no significant difference of Caspase-3 between training and control group, a definite statement about the effect of exercise on skeletal muscle apoptosis needs more studies.

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