The Study of Genetic Variability of Geographical and Host Populations of the European Corn Borer, Ostrinia nubilalis (Lepidoptera: Crambidae) by Microsatellites markers in Iran.…

Ehtesham nia, Nayer and Razmjou, Jabrael and Naseri, Bahram and gol mohamad zadeh Khiaban, Nader and Vorburger, Christoph (2016) The Study of Genetic Variability of Geographical and Host Populations of the European Corn Borer, Ostrinia nubilalis (Lepidoptera: Crambidae) by Microsatellites markers in Iran.…. Masters thesis, University of Mohaghegh Ardabili.

Text (مطالعه¬ی تنوع ژنتیکی جمعيت¬هاي جغرافيايي و میزبانی کرم ساقه¬خوار اروپائی ذرت (Lep.: Crambidae) (Hübner) Ostrinia nubilalis با نشانگرهای ریزماهواره در ایرا) Nayer Ehtesham nia.pdf Download (359kB)

Abstract

The European corn borer, Ostrinia nubilalis )Hübner( is one of the most important economic pests that could find in all parts of Iran. To study the geographical diversity, larva of this pest during 2012-2013 were collected from Ardebil, Golestan, Kordestan, East¬Azarbayjan and Sistan-Baluchestan provinces and for host diversity, from okra, melon, sugar beet, cocklebur, wheat, barley and corn from Ardabil province. By using polymerase chain reaction (PCR) and 10 different SSR primers for geographical diversity, produced 10 polymorphic sites with 100 percent polymorphism. Those markers totally produced 37 polymorphic bands in range 50 – 900 bp with mean 3.7 for every marker. Number of bands for every marker were 2 – 6. Genetic diversity analysis of the European corn borer showed significant differences within and among geographical populations and their genetic diversity based on Nei’s gene index accounted 19.2 and 80.8 percent, respectively. Maximum and minimum of genetic diversity and number of polymorphic loci were in Gorgan population 0.277, 9 and Iranshahr 0.104, 6 respectively. Maximum and minimum of heterogozysity were in Os-N3 and Os-N10 markers 0.748 and 0.203, respectively. Genetic similarity of studied geographical populations observed from 38 to 82 percent. Maximum and minimum genetic distances were observed between Sanandaj - Gorgan and Mogan - Shabstar populations 0.917, 0.198 respectively. Based on UPGMA method Mogan and Shabstar populations settled as unique in one cluster and Gorgan, Sanandaj, Iranshahr populations in another cluster. To test the correlation genetic and geographical populations distances was used Mantel test that indicated low or negative correlation (0.243 P <and r = 0.174) between genetic and geographical distances. Thus did not show correlation between increasing geographical distance in sampling sites, with their genetic distance based on SSR markers. In host populations this markers produced 32 polymorphic bands in range 50 – 900 bp with mean 3.2. Number of bands for every marker were 2 – 5. According to results, 86.92 percent of total molecular variance produced in the between populations and 13.08 percent within populations. Maximum and minimum genetic distances were observed between populations of melon - cocklebur 1.83 and wheat - barley populations 0.51. Cutting dendrogram at the maximum distance between the groups, host populations of European corn borer are attributed to two main groups that population on cocklebur in the first group and populations on okra, sugar beet, melon, wheat, barley and corn were in the second group. Second group had two subgroups, first subgroup consisted of okra, sugar beet, melon and second subgroup wheat, barley and corn.

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