A survey on relationship between cagL, orf17, cagG, and cagH genes of Helicobacter pylori cag PAI with development of gastrointestinal (GI) diseases in Iran

Raei, Negin and Negin, Saber and Latifi Navid, Saeed (2015) A survey on relationship between cagL, orf17, cagG, and cagH genes of Helicobacter pylori cag PAI with development of gastrointestinal (GI) diseases in Iran. Masters thesis, University of Mohaghegh Ardabili.

Text (بررسی ارتباط ژن‌های cagL، orf17، cagG و cagH جزیره‌ی بیماری‌زای cag هلیکوباکترپیلوری با شیوع بیماری‌های گوارشی در ایران) Negin Raei.pdf Download (1MB)

Abstract

Gastric cancer (GC) is the third common cancer that ends up death in the world. cag pathogenicity island (PAI) which contains genes associated with a more aggressive phenotype may involve in the pathogenesis of gastrointestinal disease. We aimed to examine the associations of cagH, cagL, orf17, and cagG genotypes of H. pylori cag PAI with severe gastrointestinal disease. A total of 242 H. pylori strains were genotyped. Histopathological examination and classification of subjects were performed. The frequencies of the cagH, cagL, cagG, and orf17 genotypes were 40/54 (74.1%), 53/54 (98.1%), 38/54 (70.4%), and 43/54 (79.6%) in patients with PU, respectively, while in the control group (NAG), the frequency was 87/147 (59.6%) for cagH, 121/146 (82.9%) for cagL, 109/146 (74.7%) for cagG, and 89/146 (61.0%) for orf17. The results of simple logistic regression analysis showed that the cagL and orf17 genotypes were significantly associated with an increased risk of PU not GC; the OR (95% CI) was 10.950 (1.446-82.935), and 2.504 (1.193-5.253), respectively. No significant association was found between the cagH and cagG genotypes and the risk of both the PU and the GC in Iran (P > 0.05). Finally, the multiple logistic regression analysis showed that the cagL genotype was independently and significantly associated with the age- and sex-adjusted risk for PU; the OR(95% CI) was 9.557 (1.219-17.185). We have concluded that the orf17 and especially cagL genotypes of H. pylori cag PAI could be as beneficial factors for the risk prediction of PU, but not GC in Iran. Keywords: H. pylori, cag PAI, genotype, peptic ulcerations, gastric cancer, Iran.

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