Investigation of Allelopathic effects of coumarin on lettuce from physiological - molecular and biochymical aspects

hosseinzade, hadi and Razavi, Seyed Mehdi and zahri, Saber (2015) Investigation of Allelopathic effects of coumarin on lettuce from physiological - molecular and biochymical aspects. Masters thesis, university of Mohaghegh Ardabili.

Text (بررسی اثرات آللوپاتیک کومارین برروی کاهو از برخی جنبه¬های فیزیولوژیک و مولکولی و بیوشیمیایی) hadi hosseinzade.pdf Download (337kB)

Abstract

Coumarins are regarded as a class of plant secondary metabolites of phenyl propanoid group distributed in Apiaceae family. The simple coumarin is the first compound of the class. In the present work, allelopathic potential of coumarin on the charasteric plant in allelopathic studies, Lactuca sativacv.siahoo, was investigated. At first stage, the effects of different concentration of the compound on some growth parameters of the plant such as seed germination, radicle and gemmule growth were studied. After determent of the compound optimal concentration, the germinated seeds of lettuce were cultured in peat contained pots and then were watered with Hoagland nutrition solution enriched with 2 and 10 µg/mL of coumarin. After plant growth, the effects of coumarin on some physiological and biochemical parameters were evaluated. Our results showed that lettuce seed germination was reduced by coumarin in a dose dependent manner. At the concentration of 1mg/mL the germination was inhibited entirely. Radicle and gemmule growth, wet and dried weight of roots and aerial parts of treated plants and spad chlorophyll significantly reduced by coumarin treating, as well as. However, no significant difference was recorded in chlorophyll florescence between control and coumarin treated plants. The specific activity of some antioxidant enzymes like catalase, protease and poly phenol oxidase were increased in treated plants than control, however, the activity of ascorbate peroxidase was decreased. Total protein decrease and quantitative and qualitative changes in electrophoretic pattern of aerial parts proteins were observed in treated group than control, as well as. It was concluded that coumarin as an allochemical infected lettuce of different physiological , biochemical and molecular aspects. The plant response to the stress as allochemical stress is similar to some abiotic stress such as drought or salinity.

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