Studies on MAP kinase enzyme activity in response to abiotic stresses in the cell cultures

Hasanpour, Faranak and Hamidi, Kamaledin and Zahhri, Saber and Latifi, Navid (2015) Studies on MAP kinase enzyme activity in response to abiotic stresses in the cell cultures. Masters thesis, University of Mohaghegh Ardabili.

Text Faranak Hasanpour.pdf Download (227kB)

Abstract

NK enzymes a subfamily of MAPKs include: JNK1, JNK2 and JNK3 play important rols in survival , proliferation and apoptosis in different ways .... They influence signaling pathways as well under abiotic stresses one group of genes and proteins which involved,belong to the JNK subfamily.The cancer cells use these enzyme to survive and as a result , the apoptotic pathway blocked.To study JNK genes expression and enzymes concentraition, two kinds of cancer cells including Gastric cancer cell (AGS) and Breast cancer (MCF-7) were used.They were subjected to abiotic stresses such as heat,γ and X rays radiations.In addition MTT viability assays, RT-PCR and JNK specific ELISA test were carried out to investigete apoptosis,gene expressions and JNK enzyme concentraition respectively.Results showed that abiotic stresses resulted in decrease in cell riability and increase the JNK enzyme concentraition in cancer cell lines.

Actions (login required)

View Item