Assessment of genetic diversity of purslane (Portulaca oleracea L.) accessions in Iran

khalili Baseri, Iman and Mohebodini, Mehdi and Behnamian, Mehdi and Dezhsetan, Sara (2016) Assessment of genetic diversity of purslane (Portulaca oleracea L.) accessions in Iran. Masters thesis, University of Mohaghegh Ardabili.

Text (بررسی تنوع ژنتیکی توده‌های بومی خرفه (Portulaca oleracea L.) در ایران) Iman khalili Baseri.pdf Download (1MB)

Abstract

Purslane (Portulaca oleracea L.) belongs to Portulacaceae family, an herb plant which has abounded vitamins and nutrient compounds such as fatty acids, in particular omega-3. Purslane is a highly efficient drought and salinity tolerant plant that grows in hostile environmental conditions in many countries. The study of genetic diversity is a critical component of applied plant breeding for optimizing the choice of parents in a crop breeding program. By investigating the pattern of morphological variation and application of novel molecular biological evidence we are able to test the hypothesis that morphological variation in this polymorphic species corresponds to genetic differences rather than to geographical isolation. Diversity analysis at the molecular level using PCR based markers is the efficient and rapid method of identifying the relationships and differences among the genotypes. In the present study, genetic diversity and relationships among 20 collected genotype purslane accessions were evaluated using morphological characters and ISSR markers in a randomized complete block design with 4 replications. Results of cluster analysis based on morphological traits shown that the studied genotypes classified into 9 groups. The genotyping data were used to understand the relationships among the collected accessions and identify genetically diverse genotype purslane. The 25 primers gave a total of 92 bands, among which 62 were polymorphic (67.4%). The genetic diversity as estimated by Shannon’s information index was 0.55, revealing a quite high level of genetic diversity in the germplasm. The average number of observed allele, effective allele, polymorphic information content (PIC) and Nei’s index were 2, 1.65, 0.37 and 0.37, respectively. Cluster analysis based on similarity coefficient using Unweight Pair Group Method with Arithmetic mean (UPGMA) indicated wide range of diversity across the studied accessions. There is no significant correlation between morphological and molecular traits. Keywords: genetic diversity, germplasm, primers, ISSR

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