Feeding response and digestive enzymes activity of Ephestia kuehniella (Zell.) (Lepidoptera: Pyralidae) on flour of various barley cultivars

bidar, Forough and Naseri, Bahram and Razmjou, Jabraeil and Fathi, Seyed Ali Asghar (2014) Feeding response and digestive enzymes activity of Ephestia kuehniella (Zell.) (Lepidoptera: Pyralidae) on flour of various barley cultivars. Masters thesis, university of Mohaghegh Ardabili.

Text (واكنش‌ تغذيه‌اي و فعاليت آنزيم‌هاي گوارشيEphestia kuehniella (Zell.) (Lepidoptera: Pyralida) روی آرد ارقام مختلف جو) Forough bidar.pdf Download (955kB)

Abstract

The eggs and larvae of the Mediterranean flour moth, Ephestia kuehniella (Zell.) are widely used to rear parasitoids and predators for biological control programs. In this study, nutritional responses, digestive amylolytic and proteolytic activities of the ultimate instar larvae as well as some biological parameters of E. kuehniella were studied on flour of 7 barley (Dasht, Khorram, Sahra, Reihan03, Fajr30, 5shoor and EH-83-7) and two wheat cultivars ('Bam and Sepahan') under laboratory conditions (25± 1˚C, 65± 5% R. H., and a photoperiod of 16:8 (L: D) h). The shortest larval period of ultimate instar was on barley cultivar Reihan03 (42.58± 0.69 days) and the longest was on barley cultivar EH-83-7 (53.42± 0.32 days). The longest development time was on EH-83-7 (72.12± 1.61 day) and shortest on Reihan03 (58.65± 0.87 day) and Sepahan (58.19± 1.00 day). We observed the heaviest and lightest pupal weight on 'Sepahan' (21.07± 0.77 mg) and barly cultivar Fajr30 (15.20 ± 0.58 mg). The intrinsic rate of increase (rm), was highest on wheat cultivar Sepahan (0.070 female/female/day) and lowest on barley cultivar Dasht (0.037 female/female/day). The standardized insect-growth index of E. kuehniellashowed significant difference among the barley and wheat cultivars, being highest on wheat cultivar Sepahan (0.481± 0.016 mg/day) and lowest on barly cultivar EH-83-7 (0.302± 0.011 mg/day). The highest total fecundity (224.8± 14.1eggs) was on Sepahan. Ultimate instar larvae reared on EH-83-7 showed the highest efficiency of conversion of ingested food (21.79± 0.99%). The highest general proteolytic activity was on cultivar Fajr30 (3.256± 0.250 U/mg). Also, amylolytic activity was the highest on wheat cultivar Bam (0.0262± 0.0016 mU/mg). Regarding to the obtained results, wheat cultivar Sepahan was the most suitable cultivar and barley cultivar EH-83-7 was suitable among barley cultivars for laboratory rearing of E. kuehniella.

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