Study of Protein Profile of Pea Extract and Its Biological Activity

Barari, Ladan and Asadi, Asadollah and Mitra Elmi, Maryam (2014) Study of Protein Profile of Pea Extract and Its Biological Activity. Masters thesis, University of Mohaghegh Ardabili.

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Abstract

Nowdays, despite the human has progressed in the medical and pharmaceutical sciences very much but, this progress has not been responsive to the needs of many health. In addition resistant diseases to existing drugs, especially.In proper diseases such as cancer and bacterial and fungal resistance to antibiotics each year threatends many patients lives. This has researchers to look for a more efficient alternative to drugs and antibiotics. The reason for this, this plant compound is important because these have substances with diverse biological activities such as properties of antimicrobial and anticancer. So at the thesis was taken in to consideration effect extract seed chickpea on cell line Hela, Mcf, Saos, positive and negative bacteria fungi Rhizoctonia solani. Seed chickpea was extracted by the aqueous extract and its proteins in the process of salting out was separated and its protein content was measured by the Bradford method.Then the molecular of the protein was determined by gel SDS-PAGE and electrical charge of the protein was determined by two-dimensional electrophoresis MIC and MBC method to bacteria and MFC method to the fungus and MTT assay to cell lines was used to determine the effect on the growth. The results obtained, showed that the pea extract has an inhibitory effect on bacteria, this effect on gram-positive bacteria is greater than gram-negative bacteria and also pea extract has an inhibitory effect on the Rhizoctonia solani fungi and on the cell lines was used in the thesis, that is shown the maximum inhibitory on the Hela and Mcf, the minimum effect on the saos. Therefore, the result obtained, chick pea extract as natural resource introduces wich has anti-cancer agents, antibacterial, and antifungal activity and healthy food can also was used in the pharmaceutical industry.

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