Preparation of bio scaffold and evalution of mesenchymal stem cells growth and differentiation on the scaffold

Ghahremani, Rozita and Asadi, Asadollah and Zahri, Saber (2014) Preparation of bio scaffold and evalution of mesenchymal stem cells growth and differentiation on the scaffold. Masters thesis, University of Mohaghegh Ardabili.

Text (تهیه داربست زیستی و بررسی رشد و تمایز سلول‌های بنیادی مزانشیمی بر روی آن) Rozita Ghahremani.pdf Download (432kB)

Abstract

Bone Tissue engineering is the combination of cells and a biodegradable 3D scaffold to treat deseased or damaged bone tissue. Thus, bio scaffolds derived extracellular matrix useful tool are for providing a three-dimensional environment, in order to evaluate the interaction of cells. In this regard, the bio scaffold derived from bone hindquarter fabricated by physical, chemical and Freeze drying techniques. Radiographic, calcium oxalate testing and electrolit were used to confirm dacalcification the results indicate that dacalcification have been successful every three methods. The obtained scaffold were investigated in terms of surface properties, pore size, distribution of pores and degradability behavior. Scanning electron microscopic studies showed that the scaffold surface properties are suitable. The PBS pH is also less affected in scaffold. For biocompatibility, the stem cells cultured on the scaffold and investigation viability, growth, adherence and prolifereation of the cells by invert microscopic pictures and MTT test. Finaliy, to investigate the differentiation potential of cells in the scaffold, after cultivation, were treated with osteogenic differentiation medium for 21 days. The 21 days of differentiation stained with Alizarin red were compared. Alizarin red staining show that cell was differentiation to osteoblast cells. The results obtained, suggested that the bone scaffold could be a very good choice for bone tissue engineering

Actions (login required)

View Item