Effect of Different lines and type of medium on growth of transformed hairy roots in chicory (Cichorium intybus L.)

Fathi, Roghayeh and Mohebodini, Mehdi and Chamani, Esmaeil and Farmanpour, Karim (2017) Effect of Different lines and type of medium on growth of transformed hairy roots in chicory (Cichorium intybus L.). In: 1st International and 5th National Conference on Organic vs. Conventional Agriculture, 16-17 August 2017, Ardabil, Iran.

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Abstract

Chicory (Cichorium intybus L.), is a biennial plant of the Asteraceae family, and is one of the important medicinal plants The whole of this plant (root, leaf, and seed) is used medicinally, because of the presence of inulin, chicoric acid, sesquiterpene lactones, coumarins, flavonoids and vitamins. Hairy root cultures obtained by Agrobacterium rhizogenes infection are an effective method for production of secondary metabolites, because hairy roots are genetically and biologically stable and they are able to produce metabolite within a short time and high growth rate on hormone-free medium. In this research, transgenic hairy root induction was established through Agrobacterium rhizogenes strain A4. The effects of five different line of hairy roots and three different culture media were tested to determine the best suitable for the optimum growth of hairy roots. Significant differences were shown among the 5 independent hairy root lines and different media (MS, 1/2MS and B5) for total root branching, root length and fresh weight of roots. Hairy roots were confirmed by PCR using rolB gene-specific primers. Line C produced the highest root branching (12.72 per explant) and fresh weight (3.54 g) in 1/2MS medium. Line B showed the highest root length (13.03 cm) when cultured in 1/2MS medium. The best hairy root lines, resulted in this study, can be used to improve the production of secondary metabolites of pharmaceutical values of C. intybus L. Keywords: Agrobacterium rhizogenes, Cichorium intybus L., Hairy roots, Secondary metabolites.

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