The Study Of Chloroplast Genes Sequences, MatK, RbcL and TrnL-F in Salvia nemorosa L. and Thymus syriacus Boiss

Jahangiri, Maryam (2014) The Study Of Chloroplast Genes Sequences, MatK, RbcL and TrnL-F in Salvia nemorosa L. and Thymus syriacus Boiss. Masters thesis, University of Mohaghegh Ardabili.

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Text (بررسی توالی‌های ژن¬های کلروپلاستی matk، rbcL و trnL-F در Salvia nemorosa L. و Thymus syriacus Boiss.) Maryam Jahangiri.pdf Download (1MB) | Preview

Abstract

The plant family Lamiaceae contains several species with potential therapeutic activity due to their essential oils. DNA barcoding is the use of short DNA sequences of the standard segment of the genome for large scale species identification. In this study, the chloroplast genes of two species of the Lamiaceae family, Thymus syriacus and Salvia nemorosa were sequenced to make the identification of these two species quickly and accurately. In this study, the genes matK, rbcL were used for sequencing Thymus syriacus and matK, trnL-F for Salvia nemorosa.First, total genomic DNA was extracted from leaves. Second, the nucleotide sequences of chloroplast genes were amplified and finally were sequenced. Sequenced chloroplast genes can be used to compare between species in phylogenetic and evolutionary studies and could therefor provide more accurate results within a shorter time especially in large-scale studies. The results suggest that chloroplast regions as matK, rbcL and trnL-F can be used as DNA barcodes for identification of Salvia nemorosa and Thymus syriacus species. Keywords:DNA barcoding, DNA barcode, matK, rbcL, trnL-F

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