Cloning and expression of ErbB2/HER2 protein as a biomarker for early detection of cancer

hatami tooli, Nasim (2018) Cloning and expression of ErbB2/HER2 protein as a biomarker for early detection of cancer. Masters thesis, University of Mohaghegh Ardabili.

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Text (کلون سازی و بیان پروتئین HER2 / ErbB2 به عنوان نشانگر زیستی برای تشخیص زودرس سرطان) Nasim hatami tooli.pdf Download (443kB) | Preview

Abstract

The ErbB2/HER2 is an oncogene which involves in the outbreaks of breast and ovarian cancers and has located in 17q12. The ErbB2 gene expression was intensified in the cancers and led to increment of the oncoprotein. Growth of the tumours with enhanced ErbB2 is more than other types. The ErbB2 protein has three parts, including intra cellular, inter membrane and extracellular regions. The extracellular region may be broken and releasing to the interstitial fluid and then serum. Thus, detection of this part of the protein can be used as a biomarker for identification of the cancers. The purpose of this work is cloning of ErbB2 gene in order to study on the gene properties and the recombinant protein expression which may be studied in application for the cancers' detection. So that, the vector containing of ORF of the ErbB2 gene was prepared and transferred to the CHO cell line using two optimized procedures, including the electroporation and calcium phosphate methods. The presence of the gene in the transformed cells was confirmed by PCR using BGH and T7 primers, and the expression of the gene were studied by the SDS PAGE electrophoresis.

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