Thermodynamic stability of þ-LactoglobulinA in the presence of Sugar osmolytes

Saadati, Z. and Bordbar, A. K. and Aghaei, M. and Aghaei, H. (2008) Thermodynamic stability of þ-LactoglobulinA in the presence of Sugar osmolytes. In: 11th Iranian Physical Chemistry Seminar-July 2008, July 2008, Ardabil, Iran.

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Abstract

Beta-lactoglobulin (þ-LG) is the most abundant globular protein in bovine milk. It is a globular protein with a monomer molecular weight of about 18 300 D and exists in various oligomeric states (monomer and dimers) as a function of pH, temperature, concentration, ionic strength and genetic variant. The a-helix to þ-sheet transition of proteins is a key issue for understanding the folding and biological function of a number of proteins. For example, the a‹ þ transition has been suggested to play an essential role in various conformational diseases, such as prion disease or Alzheimer disease where the a-helical forms are normal and the þ-sheet forms are amyloidogenic. Since the folding of þ-lactoglobulin is accompanied by the a‹þ transition, it will, therefore, be a useful model for clarifying the mechanism of the a‹þ transition, which is responsible for conformational diseases. Osmolytes can be polyols, sugars, polysaccharides, neutral polymers, amino acids and their derivatives, and large dipolar molecules like trimethylamine N-oxide. It is light that physicochemical properties of proteins affected from bulk properties of the solvent environment. Osmolytes as solvent additives favorably affect protein stability and solubility. While they can increase stability of proteins while protecting them from thermal denaturation, the enzymatic activity is not reduced. The thermodynamic stability of þ-LG in the presence of various amounts of osmolytes has been investigated in the presence study. Chemical denaturation by guanidium hydrochloride was used for extraction of denaturation profiles. The obtained denaturation curves were analysed on basis of two-state model and the results were interpreted in view of thermodynamics and molecular events.

Item Type: Conference or Workshop Item (Paper)
Persian Title: Thermodynamic stability of þ-LactoglobulinA in the presence of Sugar osmolytes
Persian Abstract: -
Subjects: Divisions > Conferences > 11th Iranian Physical Chemistry Seminar- July 2008
Conferences > 11th Iranian Physical Chemistry Seminar- July 2008
Divisions: Conferences > 11th Iranian Physical Chemistry Seminar- July 2008
Subjects > Conferences > 11th Iranian Physical Chemistry Seminar- July 2008
Date Deposited: 17 Jun 2019 06:07
Last Modified: 17 Jun 2019 06:07
URI: http://repository.uma.ac.ir/id/eprint/6857

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