Evaluation of genetic diversity of medicinal mushroom Ganoderma lucidum using ISSR markers

Lotfi, Shabnam and Behnamian, Mahdi and Dezhsetan, Sara and Keypour, Somayeh and Ali Hoseinzadeh, Fateme (2017) Evaluation of genetic diversity of medicinal mushroom Ganoderma lucidum using ISSR markers. Masters thesis, University of Mohaghegh Ardabili.

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Abstract

Ganoderma lucidum, is a mushroom with many medicinal properties, distributed in several regions of the country. In this study, 37 strians of G. lucidum collected from Mazandaran, Gorgan and Gilan provinces in Iran, and genetic diversity was investigated among the populations by using 21 inter-simple sequence repeat (ISSR) primers. 15 out of the 21 ISSR primers generated 173 DNA bands with 100% polymorphism. The results showed that the average genetic diversity (HS), total genetic diversity (HT) and genetic differentiation (GST) were 0.2137, 0.3629 and 0.411, respectively. The frequency of polymorphism varied from 0 to 98.27% with an average of 58.09% across all populations. Polymorphic information content (PIC) was ranged from 0.294 to 0.469 (with an average of 0.407) and Nei’s gentic diversity, Shannon index and average genetic distance were 0.214, 0.317 and 0.278, respectively. Population clustering was performed based on UPGMA method and the studied populations were divided into nine main groups. In general, the result indicated that there was a favorable genetic diversity among collected Ganoderma lucidum strains.

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