Investigation of candidate gene affeced on economic milk traits in Khalkhali goat.

Zalpour, Somayeh and Hedayat Evrigh, Nemat and Behmaram, Reza and Seyed Sharifi, Reza and Navidshad, Bahman (2017) Investigation of candidate gene affeced on economic milk traits in Khalkhali goat. Masters thesis, University of Mohaghegh Ardabili.

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Abstract

Improving indigenous populations is one of the major concerns of breeding and retention centers. Khalkhali goat is one of the most important Iranian breeds in Iran, whose economic recovery needs breeding. The purpose of this study was to detect mutations in two candidate genes (beta-lactoglobulin and POU1F1) on the economic traits of milk in khllkhali goats. To do this, a sample of 100 goats was identified and genotypes were determined using PCR-SSCP. Mutations were identified using trench sequencing and Mega 6.0 and DnaSp 5.1 software.The identified effects on the important economic traits were analyzed using SAS 9.1 software. The beta-lactoglobulin gene using sscp showed 3 different patterns and the mutations detected at positions 83 and 126 were of the type of deletion and addition that were removed at the first position and added A, and at the second position, the deletion and addition of G at the replacement position 361 A to C was observed.The mutations produced 3 haplotypes with no haplotypes having significant effect on birth weight, but haplotype 3 had significant effects on the weight of the digesting devices on the traits and components of milk, the percentage of milk fat, protein and material percentages Solid had a significant effect.The results of analyzes in the POU1F1 gene, 7 mutations, and 4 haplotypes showed that mutations detected altered adenine to cytosine (A → C), adenine to cytosine (A → C), adenine to cytosine (A → C), adenine to cytosine A → C), adenine to cytosine (A → C), adenine to cytosine (A → C), adenine to guanine (A → G), at positions 63 -133-278-291-292-807 and 916 respectively.And the produced haplotypes had a significant effect on birth weight and weaning weight, and also a significant effect on fat percentage and protein percentage was observed in the analysis of mutations in milk components.

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