Evaluation of different media effect on medicinal Ganoderma (Ganoderma lucidum) mushroom vegetative growth rate and assesment of fruit body production possibility

Zare, Bahare and Behnamian, Mahdi and Dezhsetan, Sara and Fathololomi, Solmaz (2017) Evaluation of different media effect on medicinal Ganoderma (Ganoderma lucidum) mushroom vegetative growth rate and assesment of fruit body production possibility. Masters thesis, University of Mohaghegh Ardabili.

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Abstract

Ganoderma lucidum is one of the most widely known medicinal mushrooms in the Asian countries that are used for medicinal purposes. Environmental conditions such as temperature, oxygen, carbon dioxide, moisture, light and pH affect the growth of mycelial mushrooms. In this study, in order to evaluationthe the effect of pH (4.5, 5, 5.5, 6, 6.5 and 7) on mycelial growth of two wild Ganoderma strains (GL1 and GL2), an expriment conducted as factorial based on completely randomized design with three replications. The results showed that the highest growth rate was occurred for strain GL2 (10.8 mm/day) with pH 5 and the lowest growth rate was observed for strain GL1 (8.83 mm/day) with pH 7. In order to evaluate the the effect of temperature (20, 25 and 30 °C) on mycelial growth of two wild Ganoderma strains (GL1 and GL2), an experiment was performed in factorial based on completely randomized design with three replications. The results showed that the highest growth rate was occurred for strain GL2 (14.83 mm/day) at 30 °C and the lowest growth rate was observed for strain GL1 (4.98 mm/day) at 20 °C. In general, with increasing temperature from 20 to 30 °C, the mycelial growth rate increased significantly. In order to assay the the effect of substrate type on yield, biological efficiency, fresh and dry weight and the number of cap of ganoderma lucidum a factorial experiment was conducted in a completely randomized design with three replications. The results indicated that substrate composed of wheat straw (90%) + oak sawdust (7%) + wheat bran (3%) induced the highest yield (25.8 %), biological efficiency (85.96 %), fresh weight (129 g), dry weight (91 g) and the number of cap (8.6) and the lowest yield (6.2 %), biological efficiency (20.6 %), fresh weight (31 g), dry weight (21 g) and the number of cap (2.33) were obtained from the substrate composed of wheat straw (90%) + spruce sawdust (6%) + rice bran (4%).

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