Investigation of the Relationship between babA2 and oipA Genes of Helicobacter Pylori with GastroinTestinal Diseases in Iran

Feili, Omolbanin and Zahri, Saber and Latifi Navid, Saeid (2018) Investigation of the Relationship between babA2 and oipA Genes of Helicobacter Pylori with GastroinTestinal Diseases in Iran. Masters thesis, University of Mohaghegh Ardabili.

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Abstract

Research Aim: Helicobacter pylori (H. pylori) is recognized as a causative agent of non-atrophic gastritis (NAG), peptic ulcerations (PUs), and gastric cancer (GC).The blood group antigen-binding adhesion (BabA) and outer inflammatory protein (OipA) involved in H. pyloriadherence to gastric epithelial cells have been suggested to have a role in the pathogenesis of gastrointestinal disease.We here aimed to clarify the roles of the H. pyloribabA2 and oipA genotypes in development of severe gastrointestinal disease. Research method: A total of 319 strains were obtained by culturing gastric biopsy specimens; then genomic DNA was extracted from H. pylori -positive cultures gastric biopsy specimens and genotyped by PCR. Logistic regression was used to analyze association between variables. P<0.05 was considered statistically significant. Findings: Frequency of the babA2+, andoipA+genotypes in patients with PUs was 30.8%, and 96.2%, respectively, and in non-atrophic gastritis group (NAG) was 36.6%, and 70.3%, respectively. There was no significant relationship between presence of babA2 with PUs and NAG. Results of the logistic regression analysis showed thatthe oipA+ genotype wasstronglycorrelated with the risk of PUs, the OR was 10.537[95% confidence interval (CI), 2.470-44.954, P = 0.001]. Frequency of babA2 and oipAgenes in strains obtained from patients with GC (10.5%, and 47.4%, respectively) was lower than those with NAG strains. The presence of babA2 and oipA genes showed a significant negative relationship with the risk of GC in logistic regression analysis; with OR= 0.204 (95% CI= 0.099-0.420) and 0.379 (95% CI= 0.226-0.637), respectively. Conclusion:The oipA+ genotype

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