Evaluation of Aviprin & Aviprin 3-O-Glocoside on apoptosis induction in the human cancerous prostate cell line

Moatamed, Zahra and Zahri, Saber and Rasavi, Seyed Mehdi and Hassanzadeh, Farshad (2010) Evaluation of Aviprin & Aviprin 3-O-Glocoside on apoptosis induction in the human cancerous prostate cell line. Other thesis, University of Mohaghegh Ardabili.

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Abstract

Many researchers have shown that plant-derivated polyphenolic compounds are helpful nutraceuticals in restraining various disorders such as neoplastic disease. In the present study, using preparative HPLC, two linear furanocoumarins, aviprin and Aviprin-3-O-D- glucopyranoside (A3G), were isolated from methanol extract of Prangos uloptera roots on C18 solid phase extraction cartridge. The evaluation of free radical scavenging capacity of the compounds using DPPH assay showed that aviprin is a more effective antioxidant than A3G. The biological and antiproliferative activity of the furanocoumarins were examined using human cervical carcinoma HeLa cell line and LNCaP prostatic cell line. Cell membrane integrity and cell viability were evaluated by measuring trypan blue exclusion assay and reduction of the tetrazolium-blue compound, respectively. Treating the LNCaP cell line with various concentration of the furanocoumarins showed the IC50 of aviprin and A3G 0.4 and 6.6 mg/ml, whereas their CC50 were 0.7 and 11 mg/ml, respectively. These results indicated that A3G did not significantly affect the cell line proliferation, but a comparison of IC50 and CC50 of aviprin revealed that, in the concentrations of CC50 aviprin, at least 42.7% of LNCaP cells were not dead by necrosis. Treating the HeLa cells by the furanocoumarins showed that the IC50 and CC50 of aviprin were 0.26 and 0.36 mg/ml and those of A3G were 0.33 and 0.36 mg/ml, respectively. These data point to the greater sensitivity of HeLa cell line than the LNCaP cell line, and therefore, their inhibition by necrosis could be anticipated. A morphological analysis of aviprin treated cells revealed that LNCaP cells became fragmented, whereas HeLa cells became condensed. The study of DNA fragmentation provided further evidence for the efficient inhibition of the LNCaP cell line via apoptosis induction.

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