Determination of Functional responses and biological parameters of Trichogramma sp. On eggs of Ephestia kuehniella Zeller and Plodia interpunctella (Hubner)

David, Elham and Fathi, S.A.A and Nouri Ganbalani, G (2008) Determination of Functional responses and biological parameters of Trichogramma sp. On eggs of Ephestia kuehniella Zeller and Plodia interpunctella (Hubner). Masters thesis, University of Mohaghegh Ardabili.

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Abstract

Trichogramma brassicae Bezdenko is egg parasitoid of Indianmeal moth, Plodia interpunctella (Hubner) and Ephestia kuehniella Zeller. In this research in order to study of functional response of T. brassicae on eggs of Indianmeal moth and Mill moth during no-choice experiments, different egg densities of two hosts were seperatly offered to one female of parasitoid wasp for 24 h. In no-choice experiments, T. brassicae exhibited a type III functional response to densities of eggs of two hosts. Attack rate as a function of initial egg densities (a= bN0) for T. brassicae on eggs of Indianmeal moth and Mill moth were estimated with b= 0.008 and b= 0.011 respectively and handling time were calculated 1.17 and 0.9 h, respectively. In choice experiments, combined egg densities of Indianmeal moth and Mill moth, were offered to one female of parasitoid for 24 h. T. brassicae exhibited a type II functional response on egg densities of Mill moth in present of Indianmeal moth eggs and type II functional response on egg densities of Indianmeal moth in present of Mill moth eggs. Attack rate (a) of T. brassicae on the eggs of Indian meal moth and Mill moth were calculated 0.004 and 0.006 h-1 respectively, and handling time were calculated 4.24 and 1.59 h respectively. Also, In this research, we study the effect of female parasitoid's age in their efficiency, forty eggs of Indianmeal moth and Mill moth were seperatly offered to one female of parasitoid. Eggs in each laboratory tube was renewed every 24h until the death of the parasitoid female. Also to study the effect of egg age of two hosts in parasitoid efficiency, the four age groups of egg of two hosts, including one, two, three and four day old egg baches of 40 were seperatly offered to one female of parasitoid for 24 h. The results of these experiments revealed that the number of parasitized eggs and emerged offsprings of parasitoid were decreased significantly with increasing of parasitoid age and hosts egg’s age. These results can be useful in release program of this parasitoid for control of storage pests.

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