The effect of aerobic training and intermitent hypoxia on expression angiogenic factors in cardiac male wistar rats.

Farhadi, Hassan and Siahkouhian, Marfat and Bolboli, Loltfali and Karimi, Poran (2016) The effect of aerobic training and intermitent hypoxia on expression angiogenic factors in cardiac male wistar rats. Doctoral thesis, University of Mohaghegh Ardabili.

Text (تعیین تاثیر تمرین هوازی و هیپوکسی بر میزان بیان عوامل آنژیوژنز عضله قلبی موش‌های نر نژاد ویستار) Hassan Farhadi.pdf Download (547kB)

Abstract

The aim of this study to investigate the effects of aerobic training and hypoxia on expression angiogenic factors in cardiac male wistar rats. Materials and Methods:In the current experimental study, forty male Wistar rats weighing 220±20 gr were randomly divided into four groups; normal control (NC), hypoxia (H), Hypoxia+ training(HT) and training groups. Hypoxia group exposed to chronic intermittent and Isobaric hypoxia (total pressure PiO2≈760 mmHg, 14% oxygen for 8 weeks). And exercise group ran on a treadmill (22-26 meter per min) for 8weeks, 5 session/ week with. (HT)Group, after exercise, during the day were similar to hypoxia in the hypoxic chamber. then, relative protein density of HIF1a, VEGF and P-AKT were measured with western blot method. Results: The result showed that intermittent hypoxia, exercise training+ hypoxia and exercise training significantly increased relative protein density of HIF1a, VEGF and P-AKT compared to control group (p≤0.001). also, were not significantly different found among treatment groups in relative protein density of HIF1a (p≤0.09). however, significant increased relative protein density of VEGF in hypoxia group compared to exercise training+ hypoxia group (p≤0.034). Conversely, significant increases relative protein density of P-AKT in exercise training and exercise training+ hypoxia groups compared with hypoxia group (p≤0.001). but, no significant diferrence P-AKT between in exercise training and exercise training+ hypoxia groups (p≤0.076). Conclusion:The results indicated 8 weeks exercise training, intermittent hypoxia and intervention of exercise training with hypoxia induced cardiac angiogenesis signaling pathway and activating the pathway P-AKT in male rats. It seems that hypoxia induced angiogenesis signaling pathway and exercise training is beter stimulation for activity of signaling pathway P-AKT/Akt. Also, not showed a synergistic effect of these two factors. However, according to a few studies conducted in this area, more research is needed To determine the exact mechanisms of downstream and upstream pathways. Keywords: Intermittent Hypoxia, Angiogenesis, exercise training, HIF1a, VEGF, P-AKT

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