molecular study of amylase from bacillus isolates

خياطي, خوشه and زهري, دکتر صابر and اسدي, دکتر اسداله and رضوي, دكتر سيد مهدي (2010) molecular study of amylase from bacillus isolates. Masters thesis, University of Mohaghegh Ardabili.

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Abstract

α-Amylase is an important enzyme used in the industry.Thermostable α-amylases have extensive commercial applications in starch processing, sugar production, desizing in textile industries and in detergent manufacturing processes. Amylases can be obtained from several sources. They are usually produced by bacteria belonging to the genus Bacillus for industrial applications. In this study an alkaliphilic amylase producing bacterium, Bacillus sp. KZ1, was selected from Sabalan spring isolates. This bacterium was able to produce α-amylase in the culture broth and it was purified from the culture supernatant by alginate. The molecular weight of that was determined to be 35 kDa by SDS gel electrophoresis. The enzyme was stable in the pH range 6-12 and The pH optimum for activity at optimum temperature (70˚C) was 11. The enzyme was inhibited by Ba2+, Zn2+, Mg2+, EDTA, Fe2+, H2O2, Na+, Ca2+, but the presence of Co2+, Cu2+, Mn2+enhanced enzymatic activity. The km and specific activity of that on soluble starch were 10.4 mg/ml and 992.2 U/mg, respectively.The enzyme had good biochemical characteristics such as stability in high temperatures and pH, so it could be used in industrial processes like detergents and paper industries and in medical industries.

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