Study the antitumor effects of VO-Salen complex on HeLa and McCoy cell lines

gholami rad, Farah and Zahri, Saber and bezaatpour, Abolfazl and Razavi, Seyed Mahdi and Hasanzadeh, Farshad (2010) Study the antitumor effects of VO-Salen complex on HeLa and McCoy cell lines. Other thesis, University of Mohaghegh Ardabili.

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Abstract

The presence of vanadium in biological systems, its possible physiological roles and insulin-enhancing action and anticancer activity have driven a considerable amount of research. The mineral complex of VO-Salen and 5-Br-meso-VO-Salen were synthesized and antioxidant activity of the complex was evaluated using DPPH reduction. The effects of the concentration gradients of the complexes were evaluated on HeLa and McCoy cells for 8h, 16h and 24h. The cytotoxicity and viability were evaluted by tripan blue staining and MTT reduction assay, respectively. The cytoxicity assays showed that VO-Salen is more effective than 5-Br-meso-VO-Salen. The IC50 of VO-Salen and 5-Br-meso-VO-Salen complexes on HeLa cell for 24h were 636/09 μΜ and 439/8 μΜ, respectively. These results depicted that VO-Salen and 5-Br-meso-VO-Salen complexes have significant anti oxidant capacity with RC50 654/6 and 281/7 μΜ, respectively. The apoptotic effects of the complexes were evaluated by detection of DNA fragmentation on McCoy cells. A concentration gradient and a time course effect on internucleosomal cleavage were studies. The results showed that the intensity of DNA-fragmentation was increased significantly by time and dose manner. The DNA-binding property of the complexes were evaluated using plasmid pET30 b as a template. The results showed that the VO-Salen complex was intercalated in the DNA .However, the 5-Br-meso-VO-Salen complex showed different results in the different concentrations of the complex.

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