Sublethal effects of essential oil and methanolic extract of Artemisia sieberi Besser (Asteraceae) on nutritional indices, energy reserves and digestive α-amylase activity of Trogoderma granarium Everts (Coleoptera: Dermestidae)

نوری قنبلانی, پروفسور قدیر and برزویی, مهندس احسان (2016) Sublethal effects of essential oil and methanolic extract of Artemisia sieberi Besser (Asteraceae) on nutritional indices, energy reserves and digestive α-amylase activity of Trogoderma granarium Everts (Coleoptera: Dermestidae). University of Mohaghegh Ardabili, University of Mohaghegh Ardabili.

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Abstract

The Khapra beetle, Trogoderma granarium Everts (Col.: Dermestidae), is a polyphagous and economic pest of stored grains and nuts. Plant origin products are getting maion attention in pest control due to their safety to non-target organisms. In this research, the lethal and sublethal effects of Artemisia sieberi Besser essential oil (EO) and methanolic extract was evaluated against T. granarium larvae. LC50 values after 24 h of contact and fumigant exposure with A. sieberi oil were 44.3 and 33.5, respectively. Also, LC50 value after 4 d of contact-feeding exposure with A. sieberi oil was 137.6. The EO and extract of A. sieberi produced higher than 80 percent mortalities at highest concentration. When the larval weight gain was compared, the highest value was on control, while the larvae treated with different concentrations of EO and extract showed a significant decrease in larval weight gain. Also, those larvae that were exposed to LC50 concentration of the EO (as contact or fumigant) and the extract had the lowest RCR and RGR values. The glycogen, protein and lipid content in larvae treated with LC50 concentration of the EO (as contact or fumigant) and the extract decreased compared to control. The level of glycogen, protein and lipid of larvae treated with LC50 concentration of LC50 concentration of the EO (as contact or fumigant) and the extract decreased more than LC25 concentration. The midgut α-amylase activity of 4th instar T. granarium that exposure to EO (as contact or fumigant) for 24 h and those fed on extract-containing diet for 4 days showed statistically significant reductions in comparison to control larvae. Our findings showed that A. sieberi EO and extract has significant potential for use in controlling T. granarium, in different application methods.

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