Investigating The Effect Of Chitosan On Regeneration And In VitroBulblet Production In Three Lily species

خلفی, مهسا and چمنی, اسماعیل and پوربیرامی هیر, یونس and ملکی لجایر, حسن and حسین پور آزاد, نورالدین Investigating The Effect Of Chitosan On Regeneration And In VitroBulblet Production In Three Lily species. In: 2nd International and 6th National Iranian Congress on Organic vs. Conventional Agriculture, August 2019, Ardabil, Iran.

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Abstract

In order to investigate the effects of chitosan on regeneration and in vitrobulblet production of three lily species (L. regale, L. ledebourii, L. dandie) on MS cultural medium, a completely randomized design with 10 replications were conducted in tissue culture laboratory of department of horticulture and green space of University of Mohaghegh Ardabili. Treatments consisted of five levels of zero (control), 50, 100, 150 and 200 milligrams per liter of chitosan. According to the results there was no significant difference with respect to the interaction effects between cultivar and chitosan, but the main effects of species and chitosan concentrations were significant at 5% probability. The highest percentage of regeneration was shown on media supplemented with 200 ppm of chitosan, whereas the lowest percentage of regeneration belonged to control. In general, increasing chitosan concentration on species medium from 0 to 200 ppm increased in vitrobulblet production and regeneration of lily cultivars significantly. The highest number of in vitrobulblet were recorded in L.ledeburii treated with 200 ppm of chitosan while, the lowest one observed in L. dandiespecies treated with 50 ppm of chitosan.

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