Optimization of DNA extraction method from species of Blackthorn (Prunusspinosa L.)

صفری, مهدی and قنبری, علیرضا and ترابی گیگلو, موسی and استاجی, اصغر Optimization of DNA extraction method from species of Blackthorn (Prunusspinosa L.). In: 2nd International and 6th National Iranian Congress on Organic vs. Conventional Agriculture, August 2019, Ardabil, Iran.

Text J-00126-AC.pdf Download (521kB)

Abstract

There are several methods for assessing genetic diversity in plant populations.Recently, molecular markers are widely used to study genetic diversity. For a long time, the extraction of DNA and RNA and other proteins has limitations.We can increase the quality and quantity of extracted DNA and RNA by altering the composition and amount of TBEsand how to separate the genetic material from the extraction solution.In wild plants, especially wild trees and shrubs, such as Blackthorn, It is difficult to extract high-purity genomic DNA because of high levels of polysaccharide compounds.Finally, according to the research, two methods of Vroh Bi et al. and modified CTAB for DNA extraction were selected.According to the results, the quality of the DNA extracted increased with the change in the composition of the buffer and the doubling of the amount of PVP and activated charcoal, and the amount of contaminants, especially polyphenols and polysaccharides, was significantly decreased and improved the PCR results.

Actions (login required)

View Item