The effect of hormones on callus formation in rosemary under in vitro conditions

خسروی مجدر, مهدی and پوربیرامی هیر, یونس and ترابی گیگلو, موسی and چمنی, اسماعیل and ملکی لجایر, حسن The effect of hormones on callus formation in rosemary under in vitro conditions. In: 2nd International and 6th National Iranian Congress on Organic vs. Conventional Agriculture, August 2019, Ardabil, Iran.

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Abstract

Rosemarinus officinalis L. is a plant, evergreen and perennial, very aromatic with woody stems that has many uses in medicine. This plant belongs to the mint family and its origin is reported in the Mediterranean region of southern Europe. This plant is a very rich source of bioactive phenols and this has led to its use in the manufacture of herbal medicines. Rosemary has a high nutritional value due to its many vitamins and minerals and is used in food production. It has high antioxidant properties due to its quantity and quality of polyphenolic compounds. The most important of these compounds are rosemary acid, carnosic acid, caffeic acid, thymol and carnosol. The present experiment was carried out to investigate the effect of hormones on morphological and callus formation in rosemary plant under in vitro conditions. The rosemary leaf was isolated from the terminal branch, then analyzed in MS medium containing 4 different hormonal compounds including D-4,2, TDZ, BAP and quinine using a completely randomized factorial experiment with 3 replications for 2 months. Conclusion: In this study weights and number of shoots formed in different hormonal compounds were evaluated. The results showed that the maximum amount of callus weight (6.5 mg) was 2.0 mg / l TDZ. The highest number of branches was 3.7 in TDZ at 2 mg / l.

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