Investigation callogenesisHazelnut seed in several types of basal medium

حضرتی, رقیه and زارع, ناصر Investigation callogenesisHazelnut seed in several types of basal medium. In: 2nd International and 6th National Iranian Congress on Organic vs. Conventional Agriculture, August 2019, Ardabil, Iran.

Text J-00247-AB.pdf Download (548kB)

Abstract

Taxol is used today as one of the most effective medicines in chemotherapy and is effective against a wide range of tumors and cancers. Production of secondary metabolite in in vitro culture is more profitable than extracting it from plants in nature.One of the important factors in creating optimal cell growth and production of metabolite is the composition and concentration of nutrients in the culture medium.In this study, callus induction was carried out in several basal medium. The results showed that the highest fresh callus weight (5.28gr and 5.22 gr)was obtained in ½ MS medium containing 2mg /L 2,4-D and 0.2mg /L Kin and 1 min ultrasound and in B5 medium contains 2 mg /L 2,4-D and 0.2 mg /L Kin and 150 mg /L ascorbic acid.The lowest fresh callus weight (1.32 gr) was observed in MS medium containing 2mg /L 2,4-D and 0.2mg /L Kin.The ½ MS medium provided a more suitable environment for callus growth due to its diluance and reduced salt concentration.

Actions (login required)

View Item