Inhibitory effect of two Trichoderma species on growth of some important Fusarium species in vitro

عنایتی, سمیرا and داوری, مهدی and حبیبی ینگجه, عزیز and عباداللهی, عسگر Inhibitory effect of two Trichoderma species on growth of some important Fusarium species in vitro. In: 2nd International and 6th National Iranian Congress on Organic vs. Conventional Agriculture, August 2019, Ardabil, Iran.

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Abstract

Fusarium species are among the most destructive plant pathogenic agents. Utilization of chemical toxins as a conventional method in the management of plant diseases resulted in several side-effects. Therefore, the use of bio-control strategies for management of such detrimental agents is necessary. In present study, the inhibitory effects of Trichoderma harzianum and T. viride against six species of Fusarium including F. oxysporum fsp. lycopersici (Causal agent of tomato wilt), F. oxysporum fsp. lentis (wilt of lentil), F. graminearum UM89, F. graminearum UM29 (Head blight of wheat), F. verticillioides (Ear rot of maize) and F. brasilicum (Head blight of barley) were evaluated. Experiments were conducted in two methods: dual culture of antagonist and pathogenic fungi and the effect of volatiles on the mycelial growth of pathogenic fungi in the laboratory conditions on PDA medium with three replicates and the diameter of the colonies was measured every other day. Results revealed that both of antagonistic fungi were more successful than the pathogenic fungi in nutritional competition and were able to prevent the growth of fungal mycelium. In the dual culture test, the highest inhibitory effect was related to T. viride/ F. brasilicum treatment while the lowest effect was observed in T. harzianum/ F. oxysporum interaction. Treatments related to volatile production had different results so that the most and the least inhibitory effects were observed in T. viride/ F. graminearum UM89 and T. harzianum/ F. verticillioides interactions, respectively. The overall results of this study showed that there are significant differences in interaction among the different Trichoderma species and Fusarium species; such that, primary tests on the efficacy of Trichoderma species on target plant pathogenic fungi it seems necessary before commercial usage of such products in integrated plant disease management.

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