Distribution of Diplodia bulgarica, the causal agent of apple Diplodia canker, in central regions of West Azarbaijan province

زینالی باری, رویا and ابرین بنا, مسعود and قوستا, یوبرت Distribution of Diplodia bulgarica, the causal agent of apple Diplodia canker, in central regions of West Azarbaijan province. In: 2nd International and 6th National Iranian Congress on Organic vs. Conventional Agriculture, August 2019, Ardabil, Iran.

Text I-00166-AB.pdf Download (797kB)

Abstract

The fungus Diplodia bulgarica, that belongs to the family Botryosphaeriaceae and has been described in recent years, is the causal agent of canker in various trees. This pathogen is considered as an important agent causing canker on apple trees in West Azarbaijan province but, little information is available on its distribution in central regions of the province. In this research, sampling was performed from apple orchards located in Urmia, Oshnavieh, Mahabad, Salmas, Mihammadyar and Miandoab, and fungal isolates were isolated from infected plant tissues and were then purified. Identification of the isolates were first carried out based on morphological characteristics and then using phylogenetic analysis of ITS (Internal transcribed spacers) and TEF1-α (Translation elongation factor 1-α) sequences. In total, 105 fungal isolates were obtained that 100 isolates were identified as D. bulgarica and the rest of them belonged to Cytospora parasitica, C. cincta and Clonostachys rosea The species D. bulgarica was the predominant pathogen and was distributed intensively across the studied regions however, incidence of the disease was high in the vicinity of Urmia compared with other regions.

Actions (login required)

View Item