First report of ash trees leaf spot caused by Cladosporium pseudocladosporioides in Iran

پری پور, زهرا and داوری, مهدی and عسگری, بیتا First report of ash trees leaf spot caused by Cladosporium pseudocladosporioides in Iran. In: 2nd International and 6th National Iranian Congress on Organic vs. Conventional Agriculture, August 2019, Ardabil, Iran.

Text I-00138-AB.pdf Download (531kB)

Abstract

Greenspace is important in city beautification and its diseases like leaf spot can affect the beauty of the city, parks and, streets. Besides, leaf diseases cause weakness of trees and predispose them to root diseases and decline. Ash (Fraxinus excelsior) is one of the most common trees in Ardabil greenspace and leaf spot symptoms with decline have been observed in the past few years in these trees. In this research, during summer 2018, samples from ash trees showing leaf spot were collected. In order to isolate the causal agent, infected leaves were surface sterilized with 0.5% sodium hypochlorite for 1 min and dried on sterile filter papers. Then several pieces from the edge of infected tissues were sliced and cultured on PDA at dark condition. After seven days, fungi colonies purified by single spore method. Pure cultures were identified, based on morphological characteristics including colony color, shape and growth rate on PDA and microscopic characteristics on SNA medium including conidiophores, hyphae, ramoconidia and, conidia characteristics, as Cladosporium cladosporioides. Cladosporium is one of the important genera that cause leaf spot on ash trees. Also, Fraxinus excelsior is reported as a new host for this fungus, worldwide.

Actions (login required)

View Item