Evaluation of genetic diversity in some of thornless blackberries by ISSR marker

شیری رستمی, کلثوم and مرادی, حسین and حدادی نژاد, مهدی Evaluation of genetic diversity in some of thornless blackberries by ISSR marker. In: 2nd International and 6th National Iranian Congress on Organic vs. Conventional Agriculture, August 2019, Ardabil, Iran.

Text H-00328-AC.pdf Download (493kB)

Abstract

Determination of genetic diversity of breeding material is the first step in breeding programs. Evaluation of blackberry genetic diversity is essential for breeding programs and preservation of genetic resources. In this study, genetic diversity of 10 blackberry genotypes was evaluated using 5 ISSR markers. Banding pattern based on the presence or absence of the bands showed with 0 and 1, respectively. Out of 143 fragments produced in total cultivars, 143 bands were polymorphic. . In order to evaluate the genetic similarity between cultivars, different similarity coefficient (SM, Dice and Jaccard) was calculated and Mantel corresponding test was performed. Finally, dendrogram was drawn based on Jaccard similarity coefficient and UPGMA algorithm and the Cofenetic coefficient was calculated. All genotypes formed four distinct clusters indicating the high efficiency of used primers in amplification the approximate parts of the genome. Totally, evaluation the blackberry genetic diversity using ISSR markers is suitable and ISSR marker can be used as appropriate marker system to identify the diversity and genetic relationship for breeding programs of this plant.

Actions (login required)

View Item