Evaluation of germination properties of nettle seed(Urticaceae dioica) under concentration and disinfection time

دواتگر, فاطمه and مرادی, حسین Evaluation of germination properties of nettle seed(Urticaceae dioica) under concentration and disinfection time. In: 2nd International and 6th National Iranian Congress on Organic vs. Conventional Agriculture, August 2019, Ardabil, Iran.

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Abstract

Nettle (Urtica dioca) is a member of Urticadea family and It is a perennial and Dioecious, contains a large number of secondary metabolite substance. The first step in the domestication and organic cultivation of the medicinal plant is the production of uniform and abundant seedlings.This study was done to obtain a suitable method to determine the best time and concentration of sodium hypochloride solution for surface contamination under in vitro culture. The experiment was conducted as a factorial experiment in a completely randomized design with three replications. Seed treatment was applied at different concentrations of sodium hypochloride solution and at different times in Murashige and Skoog (MS) medium. Traits such as germination rate, cotyledon length, cotyledon diameter, root number, root length, and hypocotyl length of each treatment were evaluated after 25 days. Since the seeds of wildtype plants were collected in the plant distribution areas, The results showed that in order to provide optimum seedlings and organic nettle cultivation, optimum concentration and duration of disinfection treatment, can reduce fungal and bacterial contamination in the culture medium. The highest cotyledon diameter, hypocotyl size, root number and root length were observed at 3% concentration for 5 min.

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