The Effect of different nano-elicitors on total protein content in the cell suspension culture of Galega officinalis

مینایی, سمیرا and اصغری زکریا, رسول and زارع, ناصر and خضری, مریم The Effect of different nano-elicitors on total protein content in the cell suspension culture of Galega officinalis. In: 2nd International and 6th National Iranian Congress on Organic vs. Conventional Agriculture, August 2019, Ardabil, Iran.

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Abstract

Galega officinalis is a permanent plant that used in traditional medicine for cure of type 2 Diabetes. About 20 kinds of bioactive ingredients have been extracted from G. officinalis, including, alkaloids, flavonoids, glucosides, saponines and etc. Cultivation of many plants, that produce valuable medicinal components, are hard on a large scale. Therefore, biotechnological production of attractive secondary metabolites by cell culture or plant tissue is an useful alternative to the extraction of whole plant material. Using of elicitors in plant cell and tissue culture is useful for reaching valuable components in large scale. In this study, different concentrations of iron nano oxide and molybdenum nanoparticles in two exposure time, 3 and 6 days were added to G. officinalis Cell suspension and the total protein content was evaluated. According to the result, there were significant differences in term of total protein content. Highest amounts of protein war related to 50 mg/l iron nano oxide with exposure time of 6 days.

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