Investigation of Haworthia minima organogenesis and rooting under in vitro condition

نظام دوست, محمد and کلاته جاری, سپیده and ابراهیمی, آسا Investigation of Haworthia minima organogenesis and rooting under in vitro condition. In: 2nd International and 6th National Iranian Congress on Organic vs. Conventional Agriculture, August 2019, Ardabil, Iran.

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Abstract

Haworthia minima is a small evergreen succulent plant, with hard, fleshy blue-green leaves that are covered in white tubercles. This study was carried out to investigation the organogenesis of this plant through tissue culture to determine the best hormonal composition. This experiment was conducted as a completely randomized design with 9 replications. The growth regulators examined included benzyl adenine (BA), Kintein (Kin), acetic acid (NAA) and indole butyric acid (IBA) at four levels. The results showed that kinetin and BA treatment had a significant effect on the increase in shoot production, with the difference that tissue vitrification was less affected by benzyladenin than kinetin, thus using the hormone with Concentration of 2 mg.l-1 is recommended for organogenesis of haworthia.Also the application of 0.1 mg.l-1 NAA significantly influenced rooting percentage and root length, had a good effect on the rooting of haworthia explants. Treatment with IBA at 0.5 mg.l-1 resulted in a significant increase in rooting and related factors, but this effect was less than NAA.

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