Evaluation of Haworthia minima callogenesis under in vitro condition

نظام دوست, محمد and کلاته جاری, سپیده and ابراهیمی, آسا Evaluation of Haworthia minima callogenesis under in vitro condition. In: 2nd International and 6th National Iranian Congress on Organic vs. Conventional Agriculture, August 2019, Ardabil, Iran.

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Abstract

Haworthia minima is an ornamental plant that has been widely desired due to its high resistance to inappropriate storage conditions and beautiful appearance. This plant propagation method is splitting and cuttings that in dense propagation is not respondable to market demand. For this reason, tissue culture can be used to regeneration of this plant. In order to study the callogenesis of this plant, a factorial experiment was conducted in a completely randomized design with three replications. The growth regulators included Kinetin (0, 0.1 and 1 mg.l-1), IAA (0, 0.1 and 1 mg.l-1), 2,4-D (0, 0.1 and 1 mg.l-1) and NAA (0, 0.1 and 1 mg.l-1). The studied characteristics included callus volume, fresh callus weight and relative velocity of callus growth. The results showed that the best hormonal treatments for Haworthia callogenesis was the treatment containing 0.2 mg.l-1 kinetin with 1 mg.l-1 of 2,4-D that in all three indexes led to the highest amount.

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