The effect of Nitric Oxide and Selenium Nutrition on Growth, Physiology, Biochemical Indices and Secondary Metabolites of Trigonella foenum-graecum L. under Arsenic Heavy metal Stress Conditions

Tork Zaban, Amir (2018) The effect of Nitric Oxide and Selenium Nutrition on Growth, Physiology, Biochemical Indices and Secondary Metabolites of Trigonella foenum-graecum L. under Arsenic Heavy metal Stress Conditions. Masters thesis, University of Mohaghegh Ardabili.

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Text (اثر محلول پاشی نیتریک اکسید و تغذیه با سلنیوم بر رشد، فیزیولوژی، شاخص¬های بیوشیمیایی و متابولیت¬های ثانویه گیاه شنبلیله (Trigonella foenum-graecum L.) تحت شرایط تنش عنصر سنگین آرسنیک) Amir Tork zaban.pdf Download (706kB) | Preview

Abstract

Considering the importance of vegetables in terms of fresh consumption, due to the increasing pollution of water resources and soil with heavy metals by industrial activities and the release of contaminated wastewater in nature and the necessity of controlling and counteracting it, this study coducted to reduce the disadvantage effects of arsenic contamination by nitric oxide solubilizing and selenium feeding on Fenugreek (Trigonella foenum-graecum L.). This research was conducted in two separate experiments in a factorial experiment in a completely randomized design with three replications at the University of Mohaghegh Ardabili. In the beginig, the soils were contaminated at 4 concentrations of 0, 30, 60 and 90 ppm of arsenic for 4 months. Fenugreek seeds were sowned in soil, 2 weeks after germination treated with nitric oxide at concentrations of 0, 50, 100 and 200 mM and selenium separately in concentrations of 0, 10, 20 and 40 μm, at 3 intervals of 10-days. Morphological traits including stem and root diameter, stem and root length, leaf number, shoot and root fresh weight, shoot and root dry weight, leaf area, pod number and seed number, as well as biochemical and physiological traits including: relative water content (RWC), membrane stability , Proline content, photosynthesis pigments, total carbohydrates, and activity of catalase and peroxidase enzymes were evaluated. The results showed that the arsenic control plants had the highest stem length (27.59 centimeters in the first experiment and 27.69 centimeters in the second experiment). The results of the means comparison factors showed that the highest amount of free proline in the first experiment (1.80 micrograms per gram fresh weight) was in the 90 ppm Arsenic treatment and 40 millimolar selenium treatments. In the second experiment, arsenic (90 ppm) and nitric oxide (200 micromolar) (1.80 micrograms per gram fresh weight) were observed in the combination. The highest activity of the catalase enzyme in the first experiment (0.025 changes of absorbance in milligrams of protein per minute) was observed in 90% arsenic stress regimen and the control of selenium spraying was observed. In the second experiment, Arsenic was 90 ppm and macromolecular nitric oxide (0.025 changes of absorbance in milligrams of protein per minute) was observed. Consequently, it was determined that Arcenic contamination treatments show significant harmful effects on plants, and soluble nitric oxide and nutritional supplements with selenium can both significantly reduce these effects.

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