The Effects of Dopaminergic Pathway on Mean Relative Gene Expressions of Adiponectin and Aromatase in Induced Polycystic Ovarian Syndrome Model Rats

Haghighat gollo, Khadijeh (2018) The Effects of Dopaminergic Pathway on Mean Relative Gene Expressions of Adiponectin and Aromatase in Induced Polycystic Ovarian Syndrome Model Rats. Masters thesis, University of Mohaghegh Ardabili.

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Text (بررسی اثرات مسیر دوپامینرژیکی بر میانگین بیان نسبی ژن¬های¬آدیپونکتین و آروماتاز در سندرم تخمدان پلی‌کیستیک القایی در مدل موش¬های صحرایی) Khadijeh Haghighat gollo.pdf Download (596kB) | Preview

Abstract

Polycystic ovary syndrome (PCOS) is the most common endocrine disorder in women and it is the most important causes of infertility derived of anovulation. Aromatase is an enzyme which converts testosterone to estradiol. In PCOS patients the levels of androgens increase because of the dysfunction of steroidogenesis enzymes actions and they have lower levels of aromatase enzyme activity and serum adiponectin. But the plasma insulin levels are high in PCOS patient. In the present study the effects of L-dopa and dopamine receptor antagonists were investigated on relative adiponectin and aromatase gene expressions in the ovary of PCOS model rats. Polycystic ovary syndrome was induced in Wistar female rats weighing 170-220g by intramuscular injections of estradiol valerate. The fifteen PCOS rats in three groups received saline, L-dopa(100mg/kg) or simultaneous injections of sulpride(10mg/kg), SCH23390 hydrochloride (1mg/kg) and L-dopa(100mg/kg) via intraperitoneal injection respectively. Also, five intact estrous rats as a control group received saline intraperitoneally. Mean relative adiponectin and aromatase gene expressions were determined by real time polymerase chain reaction (RT-PCR) method. The data were analyzed by SPSS software and using independent t-test and one- way ANOVA followed by post hoc Tukey test. In all cases, significance was defined by p≤0.05. The results showed that Injections of L-dopa did not significantly increase mean relative adiponectin gene expressions in the ovary in comparison to PCOS rats. But it significantly increased the mean relative ovary aromatase gene expressions in comparison to PCOS rats. Injections of l-dopa following the blocking the dopaminergic receptors by sulpride and SCH23390 caused a reduction in mean relative ovary aromatase and adiponectin gene expressions in comparison to L-dopa group but these decreases were not statistically significant. Dopaminergic signaling pathways may play an important role in controlling the disorders derived of excessive androgens levels in PCOS rats via regulating the aromatase synthesis.

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