Invigoration of deteriorated common bean seeds using priming

Saadat, Tayebeh (2017) Invigoration of deteriorated common bean seeds using priming. Masters thesis, University of Mohaghegh Ardabili.

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Abstract

In order to study on the effect of aging and priming types on French bean (Phaseolus vulgaris L. cv Sadri) seeds and gene expression of antioxidant enzymes a factorial experiment was conducted based on completely randomized design with three replications and 12 treatments including three levels of aging (control, and 78% and 88% of germination of control) and four levels of priming (control, hydro, gibberellin and salicylate priming). Aging was performed using rapid aging conditions which seeds were obtained a uniform moisture content and then stored at 45 °C to difinite time for aging. Then, seeds stored at low temperature and priming treatments applied. Results showed that interaction of aging and priming was significant on some physiological traits. The highest germination percentage observed in gibberellin primed seeds without aging which was different from control. As increasing in aging depth, there was a reduction in seed reserves using efficiency, seed reserves mobilization efficiency and fraction of used reserves, protein, phosphate and total phytin content, but priming increased these traits approximately. Respiration index and dry weight of seed residual increased as aging induction. The activity of all antioxidant enzymes decreased as increasing in aging, but priming increased their activity in comparison to the control. Real-Time PCR showed that relative gene expression of antioxidant enzymes increased in primed seeds. Peroxidase gene expression was higher than the other enzymes. In conclusion, priming of bean seeds increased the vigour of seed indirectly through the germination rate and it can impreve the establishment of seedlings in the field.

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