Investigation of genetic structure in native horses’ population using mitochondrial marker in North-west of Iran

Omri Kulankoh, Mostafa (2017) Investigation of genetic structure in native horses’ population using mitochondrial marker in North-west of Iran. Masters thesis, University of Mohaghegh Ardabili.

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Text (بررسی ساختار ژنتیکی جمعیت اسبهای شمالغرب با استفاده نشانگر میتوکندریایی) Mostafa Omri Kulankoh.pdf Download (1MB) | Preview

Abstract

The source and history of horse domestication have been studied for decades in biology and archeology. Mitochondrial genome, due to the high mutation rate, lack of recombination and maternal inheritance consider as a powerful marker in phylogenetic and phylogeographic. this study was to evaluate the genetic structure and genetic relationship between sex breeds of native horses’ using mitochondrial genome. Blood samples were collected from jugular vein 96 horse. Total DNA was extracted using DNA extraction kit(Exgene). Specific primers were used to amplify 430 bp of D-Loop region and sequenced using Sanger sequencing methods. The sequences were analyzed with bioinformatics software. The results showed that the high genetic diversity in the investigated populations. Genetic and haplotype diversity were 0.0233 and 0.980 respectively. The highest differentiate genetic was observed between Kord and Darehshori (fst=0.193) and the lowest differentiate genetic was between native horses population North-West and establ ardabil (Fst= 0.003). The lowest genetic distance (DXY= 0.02259) was observed between Arab and native horses population North-West breeds and The highest genetic distance (DXY= 0.02606) was observed between Kord and Darehshori. Tajima D 0.441 was obtained for all samples which was not significant at level 1. p> 0. horses have close genetic relationship and there are more gene fellow and genetic mixture. Keywords: Horse, Sequencing, Nucleotide diversity, diversity Genetic, D-loop,

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