Evaluating the effect of seed biopriming with Trichoderma an plant growth promoting rhizobacteria (PGPRs) on physiological and biochemical aspects of corn seedling growth under copper and aluminum stress

Afrouz, Mehdi (2017) Evaluating the effect of seed biopriming with Trichoderma an plant growth promoting rhizobacteria (PGPRs) on physiological and biochemical aspects of corn seedling growth under copper and aluminum stress. Masters thesis, university of Mohaghegh Ardabili.

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Text (ارزیابی اثر بایوپرایمینگ بذر با تریکودرما و باکتری¬های محرک رشد(PGPR) بر جنبه¬های فیزیولوژیکی و بیوشیمیایی رشد گیاهچه¬ی ذرت تحت شرایط تنش) Mehdi Afrouz.pdf Download (594kB) | Preview

Abstract

: This study was designed to evaluate the tolerance of corn plants to heavy metals as copper and aluminum, influenced by the use of Trichoderma fungi and growth stimulating bacteria. In this research, it was tried to evaluate the effects of seed biopremigation with Trichoderma fungi and growth stimulating bacteria on corn tolerance to heavy metals of copper and aluminum in a research greenhouse and laboratory suite of Faculty of Agriculture and Natural Resources, University of Mohaghegh Ardebil. This experiment was conducted as a factorial based on randomized complete block design with three replications. Experimental factors consisted of five levels of bio-priming (control, pre-treatment with Trichoderma, growth stimulator bacteria, Azospirillium and Pseudomonas) and four level of copper and aluminum treatment (control) (pH = 6.5), aluminum (concentration of 1 mM, 5 = pH), copper (200 μm, pH = 5) and aluminum + copper (pH = 5) in a hydroponic culture medium. The evaluated parameters included percentage and rate of emergence, seedling length and seed dry weight, root volume and leaf number, leaf vegetation index (SPAD), leaf relative water content (RWC), chlorophyll a and b of leaf, leaf carotenoid count , Osmotic leaf potential (OP), quantum yield (Fv / Fm), and antioxidant activity such as peroxidase and catalase. The results showed that different levels of copper and aluminum heavy metal stress had inhibitory effects on seedling percentage and seedling growth rate. Increasing the level of heavy metals of aluminum and copper was also the amount of seedling length and dry matter, root volume and leaf number, leaf vegetation index (SPAD), chlorophyll a and b leaf, leaf carotenoid content, quantum yield (Fv / Fm), and The activity of antioxidant enzymes such as peroxidase and catalase was increased, but leaf relative water content (RWC) and leaf osmotic potential (OP) of seedlings decreased. The effect of different levels of biopriming on the indices resulted in significant decrease. The values of the indices studied during the simultaneous application of copper and aluminum heavy metal stress along with the application of different levels of biopriming have a positive effect on coping and control of these stresses. It should be noted that the amount of negative effect between different levels of stress was related to the fourth level of stress (heavy metal copper and aluminum), and the highest effect of biopriming methods was on Azotopyrium and Trichoderma fungi.

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